Innate immunity is an important defense barrier for the human body. After viral pathogen-associated molecular patterns (PAMPs) are detected by host-pathogen recognition receptors (PRRs), the associated signaling pathways trigger the activation of the interferon (IFN) regulatory factor (IRF) family members and the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB). However, any gene defects among the signaling adaptors will compromise innate immune efficiency. Therefore, investigating genetic defects in the antiviral innate immune signaling pathway is important. We summarize the commonly used research methods related to antiviral immune gene defects and outline the relevant research protocols, which will help investigators study antiviral innate immunity.
Keyphrases
- innate immune
- signaling pathway
- nuclear factor
- pi k akt
- genome wide
- copy number
- toll like receptor
- dendritic cells
- epithelial mesenchymal transition
- induced apoptosis
- genome wide identification
- endothelial cells
- transcription factor
- candida albicans
- dna methylation
- sars cov
- immune response
- oxidative stress
- gene expression
- cell proliferation
- endoplasmic reticulum stress