Autologous serum protein stabilized silver quantum clusters as host-specific antibacterial agents.
Kritika SoodAsifkhan ShanavasPublished in: Nanomedicine (London, England) (2024)
Aim: To synthesize host-specific serum protein stabilized silver quantum clusters and assess their preclinical safety as potential antibacterial agents. Materials & methods: Ag-QC-NanoSera (Ag-QCNS) were synthesized using bovine, human and murine sera. Antibacterial efficacy was evaluated against E. coli (including antibiotic-resistant strain), S. aureus and P. aeruginosa . Biocompatibility, hemocompatibility and antibacterial mechanism were also investigated. Preclinical safety and biodistribution of autologous Ag-QCNS were assessed in BALB/c mice over 28 days. Results: Ag-QCNS showed high biocompatibility, hemocompatibility and high antibacterial activity at ∼12.72 μg/ml Ag equivalent. Intracellular ROS and bacterial membrane damage were confirmed as antibacterial mechanism. Ag-QCNS were established as preclinically safe. Conclusion: Ag-QCNS demonstrate potential as next-generation host-specific nanotheranostic antibacterial agents, enhancing the safety and efficacy while combating antibiotic resistance.
Keyphrases
- silver nanoparticles
- quantum dots
- highly efficient
- visible light
- cell therapy
- essential oil
- endothelial cells
- bone marrow
- gold nanoparticles
- oxidative stress
- metabolic syndrome
- molecular dynamics
- wound healing
- dna damage
- stem cells
- protein protein
- risk assessment
- amino acid
- high resolution
- energy transfer
- adipose tissue
- single molecule
- tissue engineering