EvIPqPCR, Target Circulating Tumorous Extracellular Vesicles for Detection of Pancreatic Cancer.
Komila RasulevaKeerthi Priya JangiliAlfred AkinlaluAng GuoPawel BorowiczChen-Zhong LiDali SunPublished in: Analytical chemistry (2023)
Pancreatic cancer patients predominantly present with advanced disease at diagnosis, contributing to its high mortality. A noninvasive, fast screening method to detect this disease is an unmet need. Tumor-derived extracellular vesicles (tdEVs) bearing information from parental cells have emerged as a promising cancer diagnostic biomarker. However, most tdEV-based assays have impractical sample volumes and time-consuming, complex, and costly techniques. To overcome these limitations, we developed a novel diagnostic method for pancreatic cancer screening. Our approach utilizes the mitochondrial DNA to nuclear DNA ratio of EVs as a collective cell-specific characteristic. We introduce EvIPqPCR, a fast method that combines immunoprecipitation (IP) and qPCR quantification to detect tumor-derived EVs directly from serum. Importantly, our method employs DNA isolation-free and duplexing probes for qPCR, saving at least 3 h. This technique has the potential to serve as a translational assay for cancer screening with a weak correlation to prognosis biomarkers and sufficient discriminatory power among healthy controls, pancreatitis, and pancreatic cancer cases.
Keyphrases
- mitochondrial dna
- papillary thyroid
- copy number
- induced apoptosis
- circulating tumor
- single molecule
- squamous cell
- lymph node metastasis
- type diabetes
- cell therapy
- single cell
- gene expression
- mesenchymal stem cells
- coronary artery disease
- cell proliferation
- oxidative stress
- nucleic acid
- genome wide
- young adults
- human health
- childhood cancer
- african american
- climate change
- cardiovascular disease
- high throughput sequencing