Lytic polysaccharide monooxygenases (LPMOs) have the potential to improve recalcitrant polysaccharide hydrolysis by the oxidizing cleavage of glycosidic bond. Streptomyces species are major chitin decomposers in soil ecological environments and encode multiple lpmo genes. In this study, we demonstrated that transcription of the lpmo gene, Sclpmo 10G, in the Streptomyces coelicolor A3(2) ( Sc A3(2)) strain is strongly induced by chitin. The Sc LPMO10G protein was further expressed in Escherichia coli and characterized in vitro. The Sc LPMO10G protein showed oxidation activity towards chitin. Chitinase synergy experiments demonstrated that the addition of Sc LPMO10G resulted in a substantial in vitro increase in the reducing sugar levels. Moreover, in vivo the LPMO-overexpressing strain Sc ΔLPMO10G(+) showed stronger chitin-degrading ability than the wild-type, leading to a 2.97-fold increase in reducing sugar level following chitin degradation. The total chitinase activity of Sc ΔLPMO10G(+) was 1.5-fold higher than that of Sc A3(2). In summary, Sc LPMO10G may play a role in chitin biodegradation in S. coelicolor , which could have potential applications in biorefineries.