Discovery of New Secondary Metabolites by Epigenetic Regulation and NMR Comparison from the Plant Endophytic Fungus Monosporascus eutypoides.
Zhe GuoZhong-Mei ZouPublished in: Molecules (Basel, Switzerland) (2020)
Overexpression of the histone acetyltransferase and the 1H NMR spectroscopic experiments of the endophytic fungus Monosporascus eutypoides resulted in the isolation of two new compounds, monosporasols A (1) and B (2), and two known compounds, pestaloficin C (3) and arthrinone (4). Their planar structures and absolute configurations were determined by spectroscopic analysis including high resolution electrospray ionization mass spectroscopy (HRESIMS), one-dimensional (1D) and two-dimensional (2D) NMR, and calculated electronic circular dichroism data. Compounds 1-2 were screened in cytotoxic bioassays against HeLa, HCT-8, A549 and MCF-7 cells. Our work highlights the enormous potential of epigenetic manipulation along with the NMR comparison as an effective strategy for unlocking the chemical diversity encoded by fungal genomes.
Keyphrases
- high resolution
- solid state
- cell cycle arrest
- magnetic resonance
- molecular docking
- dna methylation
- mass spectrometry
- induced apoptosis
- gene expression
- small molecule
- ms ms
- cell proliferation
- high speed
- electronic health record
- cell death
- oxidative stress
- tandem mass spectrometry
- high throughput
- machine learning
- signaling pathway
- deep learning
- molecular dynamics simulations
- risk assessment
- breast cancer cells
- single molecule