Extracellular Vesicles as Next-Generation Biomarkers in Lung Cancer Patients: A Case Report on Adenocarcinoma and Squamous Cell Carcinoma.
Monika Ruzycka-AyoushMonika Prochorec-SobieszekAndrzej CieszanowskiMaciej GlogowskiAnna Szumera-CieckiewiczJoanna PodgorskaAlicja TargonskaKamil SobczakGrażyna MosieniakIreneusz P GrudzinskiPublished in: Life (Basel, Switzerland) (2024)
Extracellular vesicles (EVs) released from primary cell lines, originating from resected tissues during biopsies in patients with non-small cell lung cancer (NSCLC) revealing adenocarcinoma and squamous cell carcinoma subtypes, were examined for membrane proteomic fingerprints using a proximity barcoding assay. All the collected EVs expressed canonical tetraspanins (CD9, CD63, and CD81) highly coexpressed with molecules such as lysosome-associated membrane protein-1 (LAMP1-CD107a), sialomucin core protein 24 (CD164), Raph blood group (CD151), and integrins (ITGB1 and ITGA2). This representation of the protein molecules on the EV surface may provide valuable information on NSCLC subtypes and offer new diagnostic opportunities as next-generation biomarkers in personalized oncology.
Keyphrases
- squamous cell carcinoma
- small cell lung cancer
- end stage renal disease
- nk cells
- prognostic factors
- gene expression
- ejection fraction
- chronic kidney disease
- lymph node metastasis
- peritoneal dialysis
- advanced non small cell lung cancer
- healthcare
- amino acid
- protein protein
- living cells
- health information
- social media
- single molecule