Polystichum braunii ameliorates airway inflammation by attenuation of inflammatory and oxidative stress biomarkers, and pulmonary edema by elevation of aquaporins in ovalbumin-induced allergic asthmatic mice.
Ammara SaleemAfza MubeenMuhammad Furqan AkhtarAlam ZebPublished in: Inflammopharmacology (2022)
Asthma is a chronic inflammation of pulmonary airways associated with bronchial hyper-responsiveness. The study was aimed to validate the folkloric use of Polystichum braunii (PB) against ovalbumin (OVA)-induced asthmatic and chemical characterization OF both extracts. Allergic asthma was developed by intraperitoneal sensitization with an OVA on days 1 and 14 followed by intranasal challenge. Mice were treated with PB methanolic (PBME) and aqueous extract (PBAE) orally at 600, 300, and 150 mg/kg and using dexamethasone (2 mg/kg) as standard from day 15 to 26. High performance liquid chromatography-diode array detector analysis revealed the presence of various bioactive compounds such as catechin, vanillic acid, and quercetin. The PBME and PBAE profoundly (p < 0.0001-0.05) declined immunoglobulin E level, lungs wet/dry weight ratio, and total and differential leukocyte count in blood and bronchial alveolar lavage fluid of treated mice in contrast to disease control. Histopathological examination showed profoundly decreased inflammatory cell infiltration and goblet cell hyperplasia in treated groups. Both extracts caused significant (p < 0.0001-0.05) diminution of IL-4, IL-5, IL-13, IL-6, IL-1β, TNF-α, and NF-κB and upregulation of aquaporins (1 and 5), which have led to the amelioration of pulmonary inflammation and attenuation of lung edema in treated mice. Both extracts profoundly (p < 0.0001-0.05) restored the activities of SOD, CAT, GSH and reduced the level of MDA dose dependently. Both extracts possessed significant anti-asthmatic action mainly PBME 600 mg/kg might be due to phenols and flavonoids and could be used as a potential therapeutic option in the management of allergic asthma.
Keyphrases
- oxidative stress
- diabetic rats
- lung function
- allergic rhinitis
- high performance liquid chromatography
- high fat diet induced
- pulmonary hypertension
- chronic obstructive pulmonary disease
- single cell
- cystic fibrosis
- dna damage
- rheumatoid arthritis
- high glucose
- cell therapy
- signaling pathway
- magnetic resonance
- stem cells
- mass spectrometry
- body mass index
- ischemia reperfusion injury
- drug induced
- induced apoptosis
- physical activity
- magnetic resonance imaging
- tandem mass spectrometry
- low dose
- computed tomography
- air pollution
- cell proliferation
- high resolution
- type diabetes
- inflammatory response
- endothelial cells
- risk assessment
- high dose
- anti inflammatory
- immune response
- ionic liquid
- simultaneous determination
- wild type
- mouse model
- cell death
- atopic dermatitis
- peripheral blood
- contrast enhanced
- liquid chromatography
- body weight