SERS- and absorbance-based catalytic assay for determination of isocarbophos using aptamer-modified FeMOF nanozyme and in situ generated silver nanoparticles.
Chongning LiFaxin YuJie YangHongyan BaiXianli MaZhiliang JiangPublished in: Mikrochimica acta (2022)
A new Fe metal-organic framework-loaded liquid crystal 4-octoxybenzoic acid (FeMOF@OCTB) nanosol was synthesized using 1,3,5-phthalic acid, ferrous sulfate, and OCTB as precursors. The FeMOF@OCTB exhibits good stability and strong catalytic effect for the polyethylene glycol 400-Ag (I) indicator reaction, which was evaluated rapidly by the slope procedure. The generated silver nanoparticles have a strong surface-enhanced Raman scattering (SERS) effect and a surface plasmon resonance absorption (Abs) peak at 420 nm. This new bimodal nanosilver indicator reaction was coupled with the isocarbophos (IPS)-aptamer (Apt) reaction. A FeMOF@OCTB nanocatalytic amplified-SERS/Abs bimodal Apt assay for IPS was established. The SERS assay can detect IPS in the concentration range 0.02-1.2 nM, with a detection limit of 0.010 nM. It has been applied to the determination of IPS in rice samples. The relative standard deviation was 4.4-5.8%, and the recovery was 97.7-104%. An Ag nanosol plasmon SERS/Abs dimode aptamer assay was fabricated for trace isocarbophos, based on highly catalysis MOF@OCTB nanoenzyme.
Keyphrases
- sensitive detection
- silver nanoparticles
- gold nanoparticles
- label free
- metal organic framework
- quantum dots
- high throughput
- loop mediated isothermal amplification
- raman spectroscopy
- photodynamic therapy
- drug delivery
- solid phase extraction
- highly efficient
- molecularly imprinted
- mass spectrometry
- crystal structure
- single cell
- magnetic nanoparticles
- oxide nanoparticles