Applications of digital PCR in COVID-19 pandemic.
Chianru TanDongdong FanNan WangFang WangBo WangLingxiang ZhuYong GuoPublished in: View (Beijing, China) (2021)
The coronavirus disease 2019 (COVID-19) pandemic has led to a public health crisis and global panic. This infectious disease is caused by a novel coronavirus named severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Digital polymerase chain reaction (dPCR), which is an emerging nucleic acid amplification technology that allows absolute quantification of nucleic acids, plays an important role in the detection of SARS-CoV-2. In this review, we introduce the principle and advantages of dPCR, and review the applications of dPCR in the COVID-19 pandemic, including detection of low copy number viruses, measurement of the viral load, preparation of reference materials, monitoring of virus concentration in the environment, detection of viral mutations, and evaluation of anti-SARS-CoV-2 drugs. We also discuss the challenges of dPCR in clinical practice.
Keyphrases
- sars cov
- respiratory syndrome coronavirus
- nucleic acid
- public health
- coronavirus disease
- copy number
- real time pcr
- label free
- loop mediated isothermal amplification
- mitochondrial dna
- clinical practice
- infectious diseases
- dna methylation
- gene expression
- mass spectrometry
- molecularly imprinted
- liquid chromatography
- drug induced