Single-cell proteomic and transcriptomic analysis of macrophage heterogeneity using SCoPE2.
Harrison SpechtEdward EmmottAleksandra A PetelskiR Gray HuffmanDavid H PerlmanMarco SerraPeter KharchenkoAntonius KollerNikolai SlavovPublished in: Genome biology (2021)
Even in a homogeneous environment, macrophage proteomes are heterogeneous. This heterogeneity correlates to the inflammatory axis of classically and alternatively activated macrophages. Our methodology lays the foundation for automated and quantitative single-cell analysis of proteins by mass spectrometry and demonstrates the potential for inferring transcriptional and post-transcriptional regulation from variability across single cells.
Keyphrases
- single cell
- rna seq
- high throughput
- mass spectrometry
- induced apoptosis
- adipose tissue
- high resolution
- cell cycle arrest
- oxidative stress
- machine learning
- liquid chromatography
- deep learning
- transcription factor
- capillary electrophoresis
- gas chromatography
- high performance liquid chromatography
- cell death
- label free
- ms ms