Colistin Sulfate Chiral Stationary Phase for the Enantioselective Separation of Pharmaceuticals Using Organic Polymer Monolithic Capillary Chromatography.
Ali FouadMontaser Sh A ShaykoonSamy M IbrahimSobhy M El-AdlAshraf GhanemPublished in: Molecules (Basel, Switzerland) (2019)
A new functionalized polymer monolithic capillary with a macrocyclic antibiotic, namely colistin sulfate, as chiral selector was prepared via the copolymerization of binary monomer mixtures consisting of glycidyl methacrylate (GMA) and ethylene glycol dimethacrylate (EGDMA) in porogenic solvents namely 1-propanol and 1,4-butanediol, in the presence of azobisiso-butyronitrile (AIBN) as initiator and colistin sulfate. The prepared capillaries were investigated for the enantioselective nano-LC separation of a group of racemic pharmaceuticals, namely, α- and β-blockers, anti-inflammatory drugs, antifungal drugs, norepinephrine-dopamine reuptake inhibitors, catecholamines, sedative hypnotics, antihistaminics, anticancer drugs, and antiarrhythmic drugs. Acceptable separation was achieved for many drugs using reversed phase chromatographic conditions with no separation achieved under normal phase conditions. Colistin sulfate appears to be useful addition to the available macrocyclic antibiotic chiral phases used in liquid chromatography.
Keyphrases
- liquid chromatography
- mass spectrometry
- capillary electrophoresis
- high resolution mass spectrometry
- ionic liquid
- tandem mass spectrometry
- escherichia coli
- simultaneous determination
- acinetobacter baumannii
- pseudomonas aeruginosa
- klebsiella pneumoniae
- gram negative
- multidrug resistant
- drug resistant
- solid phase extraction
- high performance liquid chromatography
- gas chromatography
- molecularly imprinted
- anti inflammatory drugs
- cystic fibrosis
- candida albicans
- quantum dots
- high speed
- atomic force microscopy
- water soluble