Instrumentational implementation for parallelized nanopore electrochemical measurements.
Jia-Jun WangYi-Lun YingCheng-Bing ZhongLi-Min ZhangFeng YanYi-Tao LongPublished in: The Analyst (2021)
Nanopore electrochemistry, as one of the promising tools for single molecule sensing, has proved its capability in DNA sequencing and protein analysis. To achieve a high resolution for obtaining molecular information, the nanopore electrochemical technique not only urgently requires an appropriate nanopore sensing interface with atomic resolution but also requires advanced instrumentation and its related data processing methods. In order to reveal the fundamental biological process and process the point-of-care diagnosis, it is necessary to use a nanopore sensing instrument with a high amperometric and temporal resolution as well as high throughput. The development of the instrumentation requires multi-disciplinary collaboration involving preparing a sensitive nanopore interface, low-noise circuit design, and intelligent data analysis. In this review, we have summarized the recent improvements in the nanopore sensing interface as well as discussed the higher throughput achieved by nanopore arrays and intelligent nanopore data analysis methods. The parallelized nanopore instrumentation could be popularized to all ranges of single-molecule applications.
Keyphrases
- single molecule
- data analysis
- atomic force microscopy
- living cells
- high throughput
- high resolution
- single cell
- gold nanoparticles
- mass spectrometry
- gene expression
- ionic liquid
- big data
- dna methylation
- hydrogen peroxide
- machine learning
- small molecule
- social media
- binding protein
- health information
- simultaneous determination
- liquid chromatography
- protein protein
- artificial intelligence
- tandem mass spectrometry
- high speed