Determination of minimum bactericidal concentration, in single or combination drugs, against Mycobacterium tuberculosis.
Nathally C de S SantosRegiane B de L ScodroDayane Cb LealSilvia Mt do PradoDaniela F MichelettiEloísa G SampironGiovana F CostacurtaAryadne L de AlmeidaLiliani Af da SilvaAndressa L IequeLuciana D Ghiraldi LopesRubia Af de PáduaVera Ld SiqueiraKatiany R Caleffi-FerracioliRosilene F CardosoPublished in: Future microbiology (2020)
Aim: To evaluate an assay to detect minimum bactericidal concentration (MBC) in Mycobacterium tuberculosis, using as single model rifampicin, isoniazid, levofloxacin (LVX) and linezolid (LNZ) and in combination. Material & methods: MBCs were carried out directly from resazurin microtiter assay plate and 3D checkerboard in M. tuberculosis H37Rv and five resistant clinical isolates. Results: The proposed MBC assay showed similar values to those determined by MGIT™, used as control. LVX and LNZ's MBC values were close to their MIC values. LNZ or LVX combined with isoniazid and rifampicin showed MBC value reduced in 63.7% of the assays. Conclusion: The proposed assay to determine MBCs of drugs can be applied to the study of new compounds with anti-M. tuberculosis activity to detect their bactericidal effect and also in laboratory routine for clinical dose adjustment of drugs according to the patient's profile.