Rapid assessment of viral water quality using a novel recombinase polymerase amplification test for human adenovirus.
Emily K RamesJoanne MacdonaldPublished in: Applied microbiology and biotechnology (2019)
Sensitive and rapid methods for determining viral contamination of water are critical, since illness can be caused by low numbers of viruses and bacterial indicators do not adequately predict viral loads. We developed novel rapid assays for detecting the viral water quality indicator human adenovirus (HAdV). A simple 15-min recombinase polymerase amplification step followed by a 5-min lateral flow detection is used. Species-specific assays were developed to discriminate HAdV A, B, C and F, and combined into a multiplex test (Ad-FAC). Species-specific assays enabled detection of 10-50 copies of the HAdV plasmid. Sample testing using methods optimised for wastewater analysis indicated the Ad-FAC assay showed 100% sensitivity and 100% specificity when compared with HAdV qPCR, with a detection limit as low as 50 gene copies. This is the first study to demonstrate the use of RPA for detecting enteric viruses in water samples, to assess virological water quality. The ability to rapidly detect enteric virus contamination of water could assist in more effective management of water safety and better protection of public health.
Keyphrases
- water quality
- loop mediated isothermal amplification
- high throughput
- sars cov
- public health
- real time pcr
- label free
- endothelial cells
- sensitive detection
- drinking water
- risk assessment
- escherichia coli
- induced pluripotent stem cells
- nucleic acid
- genome wide
- genetic diversity
- crispr cas
- hiv infected
- transcription factor
- wastewater treatment
- health risk
- antiretroviral therapy
- heavy metals
- disease virus