The interplay between Mn and Fe in Deinococcus radiodurans triggers cellular protection during paraquat-induced oxidative stress.
Sandra P SantosYang YangMargarida T G RosaMafalda A A RodriguesClaire Bouthier De La TourSuzanne SommerMiguel TeixeiraMaria A CarrondoPeter CloetensIsabel A AbreuCélia V RomãoPublished in: Scientific reports (2019)
The bacterium Deinococcus radiodurans is highly resistant to several stress conditions, such as radiation. According to several reports, manganese plays a crucial role in stress protection, and a high Mn/Fe ratio is essential in this process. However, mobilization of manganese and iron, and the role of DNA-binding-proteins-under-starved-conditions during oxidative-stress remained open questions. We used synchrotron-based X-ray fluorescence imaging at nano-resolution to follow element-relocalization upon stress, and its dependency on the presence of Dps proteins, using dps knockout mutants. We show that manganese, calcium, and phosphorus are mobilized from rich-element regions that resemble electron-dense granules towards the cytosol and the cellular membrane, in a Dps-dependent way. Moreover, iron delocalizes from the septum region to the cytoplasm affecting cell division, specifically in the septum formation. These mechanisms are orchestrated by Dps1 and Dps2, which play a crucial role in metal homeostasis, and are associated with the D. radiodurans tolerance against reactive oxygen species.
Keyphrases
- fluorescence imaging
- oxidative stress
- reactive oxygen species
- metal organic framework
- single molecule
- photodynamic therapy
- stress induced
- single cell
- dna damage
- high resolution
- peripheral blood
- oxide nanoparticles
- room temperature
- minimally invasive
- hydrogen peroxide
- radiation therapy
- risk assessment
- computed tomography
- cell free
- adverse drug
- mass spectrometry
- mesenchymal stem cells
- induced apoptosis
- heat stress
- endoplasmic reticulum stress