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VASH1-SVBP and VASH2-SVBP generate different detyrosination profiles on microtubules.

Sacnicte Ramirez-RiosSung Ryul ChoiChadni SanyalThorsten B BlumChristophe BoscFatma KrichenEric DenarierJean-Marc SoleilhacBéatrice BlotCarsten JankeVirginie Stoppin-MelletMaria M MagieraIsabelle ArnalMichel O SteinmetzMarie Jo Moutin
Published in: The Journal of cell biology (2022)
The detyrosination/tyrosination cycle of α-tubulin is critical for proper cell functioning. VASH1-SVBP and VASH2-SVBP are ubiquitous enzymes involved in microtubule detyrosination, whose mode of action is little known. Here, we show in reconstituted systems and cells that VASH1-SVBP and VASH2-SVBP drive the global and local detyrosination of microtubules, respectively. We solved the cryo-electron microscopy structure of VASH2-SVBP bound to microtubules, revealing a different microtubule-binding configuration of its central catalytic region compared to VASH1-SVBP. We show that the divergent mode of detyrosination between the two enzymes is correlated with the microtubule-binding properties of their disordered N- and C-terminal regions. Specifically, the N-terminal region is responsible for a significantly longer residence time of VASH2-SVBP on microtubules compared to VASH1-SVBP. We suggest that this VASH region is critical for microtubule detachment and diffusion of VASH-SVBP enzymes on lattices. Our results suggest a mechanism by which VASH1-SVBP and VASH2-SVBP could generate distinct microtubule subpopulations and confined areas of detyrosinated lattices to drive various microtubule-based cellular functions.
Keyphrases
  • electron microscopy
  • high resolution
  • induced apoptosis
  • cell death
  • oxidative stress
  • mass spectrometry
  • cell proliferation
  • single cell