On the Mediated Electron Transfer of Immobilized Galactose Oxidase for Biotechnological Applications.
Fangyuan ZhaoAnn Cathrin BrixAnna LielpetereWolfgang SchuhmannFelipe ConzueloPublished in: Chemistry (Weinheim an der Bergstrasse, Germany) (2022)
The use of enzymes as catalysts in chemical synthesis offers advantages in terms of clean and highly selective transformations. Galactose oxidase (GalOx) is a remarkable enzyme with several applications in industrial conversions as it catalyzes the oxidation of primary alcohols. We have investigated the wiring of GalOx with a redox polymer; this enables mediated electron transfer with the electrode surface for its potential application in biotechnological conversions. As a result of electrochemical regeneration of the catalytic center, the formation of harmful H 2 O 2 is minimized during enzymatic catalysis. The introduced bioelectrode was applied to the conversion of bio-renewable platform materials, with glycerol as model substrate. The biocatalytic transformations of glycerol and 5-hydroxymethylfurfural (HMF) were investigated in a circular flow-through setup to assess the possibility of substrate over-oxidation, which is observed for glycerol oxidation but not during HMF conversion.
Keyphrases
- electron transfer
- stem cells
- atomic force microscopy
- hydrogen peroxide
- high throughput
- ionic liquid
- wastewater treatment
- gold nanoparticles
- highly efficient
- amino acid
- nitric oxide
- mass spectrometry
- risk assessment
- structural basis
- crystal structure
- liquid chromatography
- metal organic framework
- molecularly imprinted
- solid phase extraction