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A bacterial assay for rapid screening of IAA catabolic enzymes.

Federica BrunoniSilvio CollaniJan ŠimuraMarkus SchmidCatherine BelliniKaren Ljung
Published in: Plant methods (2019)
This new method represents an alternative to existing in vitro assays. It can be applied to the analysis of IAA metabolites that are produced upon supplementation of substrate to engineered bacterial cultures and can be used for a rapid screening of orthologous candidate genes from non-model species.
Keyphrases
  • high throughput
  • loop mediated isothermal amplification
  • ms ms
  • amino acid