Characterizing the Spatiotemporal Transcriptomic Response of the Right Ventricle to Acute Pressure Overload.
Vitaly O KheyfetsSushil KumarPaul M HeerdtKenzo IchimuraR Dale BrownMelissa LuceroIlham EssafriSarah WilliamsKurt R StenmarkEdda SpiekerkoetterPublished in: International journal of molecular sciences (2023)
This study analyzed microarray data of right ventricular (RV) tissue from rats exposed to pulmonary embolism to understand the initial dynamic transcriptional response to mechanical stress and compare it with experimental pulmonary hypertension (PH) models. The dataset included samples harvested from 55 rats at 11 different time points or RV locations. We performed principal component analysis (PCA) to explore clusters based on spatiotemporal gene expression. Relevant pathways were identified from fast gene set enrichment analysis using PCA coefficients. The RV transcriptomic signature was measured over several time points, ranging from hours to weeks after an acute increase in mechanical stress, and was found to be highly dependent on the severity of the initial insult. Pathways enriched in the RV outflow tracts of rats at 6 weeks after severe PE share many commonalities with experimental PH models, but the transcriptomic signature at the RV apex resembles control tissue. The severity of the initial pressure overload determines the trajectory of the transcriptomic response independent of the final afterload, but this depends on the location where the tissue is biopsied. Chronic RV pressure overload due to PH appears to progress toward similar transcriptomic endpoints.
Keyphrases
- mycobacterium tuberculosis
- pulmonary embolism
- single cell
- gene expression
- pulmonary hypertension
- rna seq
- liver failure
- drug induced
- pulmonary artery
- inferior vena cava
- dna methylation
- pulmonary arterial hypertension
- intensive care unit
- stress induced
- aortic dissection
- machine learning
- oxidative stress
- left ventricular
- heart failure
- heat stress