Mesoporous silica nanoparticles loaded with fluorescent coumarin-5-fluorouracil conjugates as mitochondrial-targeting theranostic probes for tumor cells.
Xiaojing YangDi-Feng ChenLin-Song LiXue-Jie ZhaoMei-Xia ZhaoPublished in: Nanotechnology (2021)
In this study, a nanodrug carrier (mesoporous silica nanoparticle (MSN)-SS-cysteamine hydrochloride (CS)-hyaluronic acid (HA)) for targeted drug delivery was prepared using MSNs, in which HA was used as a targeting ligand and blocking agent to control drug release. Coumarin is a fluorescent molecule that targets mitochondria. Two conjugates (XDS-DJ and 5-FUA-4C-XDS) were synthesized by chemically coupling nitrogen mustard and 5-fluorouracil with coumarin, which was further loaded into MSN-SS-CS-HA nanocarriers. MTT analysis demonstrated that the nanocomposite MSN-SS-CS@5-FUA-4C-XDS/HA displayed stronger cytotoxicity toward HCT-116 cells than HeLa or QSG-7701 cells. Furthermore, MSN-SS-CS@5-FUA-4C-XDS/HA was able to target the mitochondria of HCT-116 cells, causing decreased mitochondrial membrane potential and excessive production of reactive oxygen species. These results indicate that MSN-SS-CS@5-FUA-4C-XDS/HA has the potential to be a nanodrug delivery system for the treatment of colon cancer.
Keyphrases
- drug delivery
- cancer therapy
- cell cycle arrest
- induced apoptosis
- drug release
- cell death
- reactive oxygen species
- hyaluronic acid
- quantum dots
- oxidative stress
- endoplasmic reticulum stress
- pi k akt
- signaling pathway
- fluorescence imaging
- photodynamic therapy
- living cells
- body mass index
- physical activity
- weight gain
- room temperature
- wound healing
- gold nanoparticles
- human health
- replacement therapy