Amino acid supplementation confers protection to red blood cells prior to Plasmodium falciparum bystander stress.
Heather Colvin BinnsElmira AlipourCameron E SherlockDinah S NahidJohn F WhitesidesAnderson O'Brien CoxCristina M FurduiGlen S MarrsDaniel B Kim-ShapiroRegina Joice CordyPublished in: Blood advances (2024)
Malaria is a highly oxidative parasitic disease in which anemia is the most common clinical symptom. A major contributor to malarial anemia pathogenesis is the destruction of bystander, uninfected red blood cells (RBCs). Metabolic fluctuations are known to occur in the plasma of individuals with acute malaria, emphasizing the role of metabolic changes in disease progression and severity. Here, we report that conditioned media from Plasmodium falciparum culture induces oxidative stress in uninfected, catalase-depleted RBCs. As cell permeable precursors to glutathione, we show a benefit of pre-exposure to exogenous glutamine, cysteine, and glycine (QCG) amino acids for RBCs and that this pre-treatment intrinsically prepares RBCs to mitigate oxidative stress.
Keyphrases
- plasmodium falciparum
- red blood cell
- amino acid
- oxidative stress
- hiv infected
- chronic kidney disease
- dna damage
- iron deficiency
- liver failure
- diabetic rats
- single cell
- induced apoptosis
- cell therapy
- drug induced
- antiretroviral therapy
- stem cells
- mesenchymal stem cells
- intensive care unit
- endoplasmic reticulum stress
- bone marrow
- acute respiratory distress syndrome
- extracorporeal membrane oxygenation
- mechanical ventilation