PB1F2 from Influenza A Virus Regulates the Interaction between Cytochrome C and Cardiolipin.
Yujuan WangJunfeng WangPublished in: Membranes (2022)
PB1F2 is a membrane associated protein encoded by the influenza virus gene in the host. Similar to endogenous pro-apoptotic proteins, it acts on the mitochondria of the host immune cells, inducing apoptosis of the cells. The PB1F2 protein has been demonstrated to facilitate the release of cytochrome c in addition to impairing the integrity of the inner mitochondrial membrane. This investigation focused on how the protein PB1F2 interacted with cardiolipin and cytochrome c. The regulation of PB1F2 on the binding of cytochrome c to cardiolipin in two kinds of in vitro membrane mimics was investigated by biophysical techniques. PB1F2 aids in the dissociation of cytochrome c-cardiolipin complexes in liposomes and nanodiscs. The results provide novel explanations and evidence for how PB1F2 functions as a viral virulence factor by inducing immune cell death.
Keyphrases
- escherichia coli
- heavy metals
- cell death
- cell cycle arrest
- aqueous solution
- oxidative stress
- risk assessment
- induced apoptosis
- drug delivery
- sars cov
- binding protein
- endoplasmic reticulum stress
- anti inflammatory
- amino acid
- transcription factor
- pseudomonas aeruginosa
- dna binding
- atomic force microscopy
- antimicrobial resistance
- single molecule
- high speed