Chitosan/Gelatin Scaffolds Loaded with Jatropha mollissima Extract as Potential Skin Tissue Engineering Materials.
Matheus Ferreira de SouzaHenrique Nunes da SilvaJosé Filipe Bacalhau RodriguesMaria Dennise Medeiros MacêdoWladymyr Jefferson Bacalhau de SousaRossemberg Cardoso BarbosaMarcus Vinícius Lia FookPublished in: Polymers (2023)
This work aimed to develop chitosan/gelatin scaffolds loaded with ethanolic extract of Jatropha mollissima (EEJM) to evaluate the influence of its content on the properties of these structures. The scaffolds were prepared by freeze-drying, with different EEJM contents (0-10% ( w / w )) and crosslinked with genipin (0.5% ( w / w )). The EEJM were characterized through High Performance Liquid Chromatography coupled to a Diode Array Detector (HPLC-DAD), and the determination of three secondary metabolites contents was accomplished. The physical, chemical and biological properties of the scaffolds were investigated. From the HPLC-DAD, six main substances were evidenced, and from the quantification of the total concentration, the condensed tannins were the highest (431.68 ± 33.43 mg·g -1 ). Spectroscopy showed good mixing between the scaffolds' components. Adding and increasing the EEJM content did not significantly influence the properties of swelling and porosity, but did affect the biodegradation and average pore size. The enzymatic biodegradation test showed a maximum weight loss of 42.89 within 28 days and reinforced the efficiency of genipin in crosslinking chitosan-based materials. The addition of the extract promoted the average pore sizes at a range of 138.44-227.67 µm, which is compatible with those reported for skin regeneration. All of the scaffolds proved to be biocompatible for L929 cells, supporting their potential application as skin tissue engineering materials.
Keyphrases
- tissue engineering
- wound healing
- ms ms
- high performance liquid chromatography
- simultaneous determination
- drug delivery
- solid phase extraction
- tandem mass spectrometry
- high resolution
- soft tissue
- mass spectrometry
- weight loss
- oxidative stress
- hyaluronic acid
- induced apoptosis
- cancer therapy
- mental health
- magnetic resonance imaging
- cell death
- bariatric surgery
- cell cycle arrest
- anti inflammatory
- type diabetes
- high throughput
- nitric oxide
- magnetic resonance
- body mass index
- drug release
- gastric bypass
- drinking water
- ionic liquid
- insulin resistance
- risk assessment
- climate change
- obese patients
- high speed