SUGAR-seq enables simultaneous detection of glycans, epitopes, and the transcriptome in single cells.
Conor J KearneyStephin J VervoortKelly M RamsbottomIzabela TodorovskiEmily J LelliottMagnus ZethovenLizzy PijpersBen P MartinTimothy SempleLuciano G MartelottoJoseph A TrapaniIan A ParishNichollas E ScottJane OliaroRicky W JohnstonePublished in: Science advances (2021)
Multimodal single-cell RNA sequencing enables the precise mapping of transcriptional and phenotypic features of cellular differentiation states but does not allow for simultaneous integration of critical posttranslational modification data. Here, we describe SUrface-protein Glycan And RNA-seq (SUGAR-seq), a method that enables detection and analysis of N-linked glycosylation, extracellular epitopes, and the transcriptome at the single-cell level. Integrated SUGAR-seq and glycoproteome analysis identified tumor-infiltrating T cells with unique surface glycan properties that report their epigenetic and functional state.
Keyphrases
- single cell
- rna seq
- high throughput
- cell surface
- loop mediated isothermal amplification
- induced apoptosis
- gene expression
- real time pcr
- label free
- dna methylation
- cell cycle arrest
- high resolution
- pain management
- oxidative stress
- transcription factor
- signaling pathway
- small molecule
- genome wide
- chronic pain
- high density
- heat shock
- deep learning
- sensitive detection
- heat stress