Formate Oxidase (FOx) from Aspergillus oryzae: One Catalyst Enables Diverse H2 O2 -Dependent Biocatalytic Oxidation Reactions.
Florian TievesSébastien Jean-Paul WillotMorten Martinus Cornelis Harald van SchieMarine Charlène Renée RauchSabry Hamdy Hamed YounesWuyuan ZhangJiaJia DongPatricia Gomez de SantosJohn Mick RobbinsBettina BommariusMiguel AlcaldeAndreas Sebastian BommariusFrank HollmannPublished in: Angewandte Chemie (International ed. in English) (2019)
An increasing number of biocatalytic oxidation reactions rely on H2 O2 as a clean oxidant. The poor robustness of most enzymes towards H2 O2 , however, necessitates more efficient systems for in situ H2 O2 generation. In analogy to the well-known formate dehydrogenase to promote NADH-dependent reactions, we here propose employing formate oxidase (FOx) to promote H2 O2 -dependent enzymatic oxidation reactions. Even under non-optimised conditions, high turnover numbers for coupled FOx/peroxygenase catalysis were achieved.