Time dependent effect of cold ischemia on the phosphoproteome and protein kinase activity in fresh-frozen colorectal cancer tissue obtained from patients.

Tineke E BuffartRosanne A H M van den OordAdriënne van den BergRiet HilhorstNiek BastiaensenHans F M PruijtAdriaan van den BrulePeet NooijenMariette LabotsRichard R de Goeij-de HaasHenk DekkerSander R PiersmaThang V PhamTheo van der LeijRik de WijnRob RuijtenbeekConnie R JiménezHenk M W Verheul

Published in: Clinical proteomics (2021)

The vast majority of the phosphoproteome as well as the activity of protein kinases in colorectal cancer resection tissue is stable up to 180 min of CIT and reflects tumor characteristics. However, specific changes in kinase activity with increasing CIT were observed. Therefore, stringent tissue collection procedures are advised to minimize changes in kinase activity during CIT.

Keyphrases

protein kinase
end stage renal disease
newly diagnosed
peritoneal dialysis
binding protein