Optimization of sample preparation for intact cell mass spectrometry (matrix-assisted laser desorption/ionization linear time-of-flight mass spectrometry) of endophytic Xylaria.

Luciana da Silva AmaralEdson Rodrigues-FilhoChristian P KubicekChristoph HerwigMartina Marchetti-DeschmannGuenter Allmaier

Published in: Rapid communications in mass spectrometry : RCM (2018)

X. arbuscula could be analyzed by ICMS. However, an extraction step was required to provide suitable MALDI mass spectra. Formic acid-, AB- and thymol-containing solutions were demonstrated to be good cocktails for the extraction of peptide/protein biomarkers from these fungi.

Keyphrases

mass spectrometry
liquid chromatography
single cell
gas chromatography
high performance liquid chromatography
capillary electrophoresis
cell therapy
high resolution
protein protein
stem cells
molecularly imprinted
density functional theory
small molecule
tandem mass spectrometry
bone marrow
solid state