ALKBH5-induced demethylation of mono- and dimethylated adenosine.

Timm T EnsfelderMatthias Q Kurz Katharina IwanSimon GeigerSarah MatheislMarkus MüllerRoland BeckmannThomas Carell

Published in: Chemical communications (Cambridge, England) (2018)

RNA contains methylated A-base derivatives. A methylation to m6A and then demethylation regulate homeostasis in mRNA. It is assumed that m6A is mainly demethylated by the α-ketoglutarate dependent oxidase ALKBH5. Here we show that ALKBH5 also demethylates the dimethylated adenosine m62A, which is a non-canonical base present in ribosomal RNA.

Keyphrases

protein kinase
high glucose
diabetic rats
nucleic acid
dna methylation
genome wide
drug induced
gene expression
stress induced