Digital Quantification of Intratumoral CD8+ T-Cells Predicts Relapse and Unfavorable Outcome in Uveal Melanoma.
Ozge HurdoganFrancesco De LoguFrancesca GalliSamuray TuncerFilippo UgoliniSara SimiFrancesca PortelliRomina NassiniDaniela MassiNesimi BuyukbabaniPublished in: Cancers (2022)
Although it is a disease that occurs mainly in the Caucasian population, uveal melanoma (UM) is the most common primary intraocular tumor in adults. Here, we used digital pathology and image analysis for the diagnosis of UM and the prediction of the prognosis. Our retrospective study included a total of 404 histopathological slides from 101 patients. A digital image acquisition and quantitative analysis of tissue immune biomarkers (CD4, CD8, CD68, CD163) were performed. A negative impact of the intratumoral CD8 positive cell density higher than 13.3 cells/mm 2 was detected for both RFS (HR 2.08, 95% Cl 1.09 to 3.99, p = 0.027) and OS (HR 3.30, 95% CI 1.58 to 6.88, p = 0.001). Moreover, we confirmed that older age and stage III were independent negative prognostic factors for both RFS and OS. Our results suggest that a specific distribution profile of CD8 in UM might predict the risk of relapse and death, with potential implications for determining which subgroups of UMs are amenable to specific pharmacological treatment regimens.
Keyphrases
- prognostic factors
- end stage renal disease
- induced apoptosis
- ejection fraction
- newly diagnosed
- chronic kidney disease
- peritoneal dialysis
- physical activity
- single cell
- free survival
- deep learning
- cell therapy
- nk cells
- mesenchymal stem cells
- human health
- skin cancer
- mass spectrometry
- community dwelling
- combination therapy