Photobiomodulation and estrogen stabilize mitochondrial membrane potential in angiotensin-II challenged porcine aortic smooth muscle cells.
Lilach GavishDan GilonRonen BeeriAyelet ZuckermanDean NachmanS David GertzPublished in: Journal of biophotonics (2020)
Rupture of Abdominal aortic aneurysm (AAA) is among the 15 leading causes of death after age 65. Using high frequency ultrasound, we showed that photobiomodulation (PBM) prevents formation and progression of AAA in the angiotensin-II (Ang-II)-infused, apolipoprotein-e-deficient mouse model. In the current study we report that while challenge of porcine aortic Smooth Muscle Cells (SMCs) with Ang-II (1 μM) resulted in a marked decay in mitochondrial membrane potential (MitMP) vs non-challenged cells, treatment with PBM (continuous diode laser, 780 nm, 6.7 mW/cm2 , 5 minutes, 2 J/cm2 ) or pre-incubation with estrogen (50 nM, 1 hour) significantly attenuated this deterioration in MitMP. We also report that PBM and estrogen markedly affected porcine aortic SMC contraction and modified mitochondrial dispersion reflecting important influence on SMC function. These studies provide strong evidence of the important underlying role of mitochondria in the preventive effect of PBM on formation and progression of AAA and its reduced incidence and delayed onset in women.
Keyphrases
- angiotensin ii
- high frequency
- aortic valve
- angiotensin converting enzyme
- abdominal aortic aneurysm
- vascular smooth muscle cells
- oxidative stress
- mouse model
- estrogen receptor
- transcranial magnetic stimulation
- left ventricular
- pulmonary artery
- induced apoptosis
- photodynamic therapy
- aortic dissection
- magnetic resonance imaging
- blood pressure
- risk factors
- polycystic ovary syndrome
- coronary artery
- wound healing
- type diabetes
- heart failure
- cell death
- computed tomography
- human health
- mass spectrometry
- signaling pathway
- pregnancy outcomes
- ultrasound guided
- smooth muscle
- endoplasmic reticulum
- high speed
- insulin resistance
- cervical cancer screening