Evaluation of the Induction of Cell-Mediated Immunity Against Candida albicans in a Model of Cutaneous Infection in Newborn 0-Day-Old Mice.
O E Flores-MaldonadoA M MontoyaA AndradeG M GonzálezS A Aguilar-FernándezM Elizondo-ZertucheR Chacón-SalinasH Rocha-RodríguezMiguel A Becerril-GarcíaPublished in: Mycopathologia (2019)
Candida albicans is a commensal fungus of the skin and mucous membranes in humans, but it is also responsible for mucocutaneous and systemic infections in immunocompromised patients like low birth weight neonates and premature newborns. The epicutaneous application of C. albicans is widely used to study the immune response against this pathogen in adult mice models. However, the immune response of newborns against infections caused by the genus Candida is poorly understood. In order to mimic premature human infection, we developed a model of C. albicans epicutaneous infection in newborn mice. We found that yeasts were able to colonize while the pseudohyphae invaded the epidermis. Recruitment of polymorphonuclear and mononuclear cells at the infection zone was observed. Fungal invasion, fungal burden and cellular infiltration displayed a time- and dose-dependent response. Interestingly, newborn mice were able to control C. albicans primary infection. Finally, we showed that the epicutaneous infection of C. albicans in newborn mice at birth results in the induction of cell-mediated immunity as evinced by delayed-type hypersensitivity assays.
Keyphrases
- candida albicans
- biofilm formation
- low birth weight
- immune response
- high fat diet induced
- preterm infants
- pregnant women
- single cell
- type diabetes
- stem cells
- cell therapy
- ejection fraction
- induced apoptosis
- metabolic syndrome
- young adults
- newly diagnosed
- adipose tissue
- insulin resistance
- dendritic cells
- prognostic factors
- signaling pathway
- staphylococcus aureus
- wound healing
- soft tissue
- pregnancy outcomes
- drug induced
- acute respiratory distress syndrome
- saccharomyces cerevisiae