TGFβ-induced osteogenic potential of human amniotic fluid stem cells via CD73-generated adenosine production.
Kwan-Leong HauAnna Maria RanzoniFilipa VlahovaKate HawkinsPaolo De CoppiAnna L DavidAnna Louise DavidPublished in: Scientific reports (2017)
The human amniotic fluid stem cell (hAFSC) population consists of two morphologically distinct subtypes, spindle-shaped and round-shaped cells (SS-hAFSCs and RS-hAFSCs). Whilst SS-hAFSCs are routinely expanded in mesenchymal-type (MT) conditions, we previously showed that they acquire broader differentiation potential when cultured under embryonic-type (ET) conditions. However, the effects of culture conditions on RS-hAFSCs have not been determined. Here, we show that culturing RS-hAFSCs under ET conditions confers faster proliferation and enhances the efficiency of osteogenic differentiation of the cells. We show that this occurs via TGFβ-induced activation of CD73 and the associated increase in the generation of extracellular adenosine. Our data demonstrate that culture conditions are decisive for the expansion of hAFSCs and that TGFβ present in ET conditions causes the phenotype of RS-hAFSCs to revert to an earlier state of stemness. Cultivating RS-hAFSCs in ET conditions with TGFβ may therefore increase their therapeutic potential for clinical applications.
Keyphrases
- stem cells
- endothelial cells
- transforming growth factor
- induced apoptosis
- bone marrow
- mesenchymal stem cells
- cell cycle arrest
- diabetic rats
- epithelial mesenchymal transition
- signaling pathway
- climate change
- cell death
- induced pluripotent stem cells
- machine learning
- electronic health record
- artificial intelligence
- human health
- pi k akt