Antimicrobial efficacy and biocompatibility of extracts from Cryptocarya species.
Jacqueline de Oliveira ZoccolottiAlberto José CavalheiroCamilla Olga TassoBeatriz Ribeiro RibasTúlio Morandin FerrisseJanaina Habib JorgePublished in: PloS one (2021)
This study evaluated the efficacy of Cryptocarya spp extracts on biofilm of Candida albicans and its biocompatibility. Mature biofilm of C. albicans was formed on denture base acrylic resin samples and the fungicidal effect of the extracts was evaluated by Alamar Blue® assay, counting colony-forming units (CFU/mL) and confocal laser scanning microscopy (CLSM). Cytotoxicity of extracts from Cryptocarya species was evaluated by AlamarBlue® assay, using normal oral keratinocytes (NOK) cells. In additional, Analysis of plant extracts by ultra-high-performance liquid chromatography-diode array detector-tandem mass spectrometry (UPLC-DAD-MS) was performed. The results showed significant reduction in the cellular metabolism and in the number of CFU/mL of C. albicans (p<0.05). The concentration of 0.045 g/mL completely inhibited the number of CFU/mL. Regarding cytotoxicity, all extracts decreased cell viability compared to the control group. CLSM analysis showed predominance of live cells, but with a great difference between the groups. Antimicrobial activity of extracts from Cryptocarya on C. albicans biofilm was confirmed. However, all extracts showed toxicity on NOK cells.
Keyphrases
- candida albicans
- tandem mass spectrometry
- ultra high performance liquid chromatography
- biofilm formation
- simultaneous determination
- induced apoptosis
- staphylococcus aureus
- pseudomonas aeruginosa
- high resolution
- cell cycle arrest
- high throughput
- liquid chromatography
- mass spectrometry
- high performance liquid chromatography
- ms ms
- oxidative stress
- magnetic resonance imaging
- magnetic resonance
- solid phase extraction
- cystic fibrosis
- optical coherence tomography
- cell death
- cell proliferation
- high speed