H19 Sperm Methylation in Male Infertility: A Systematic Review and Meta-Analysis.
Rossella CannarellaAndrea CrafaFederica BarbagalloScott D LundySandro La VigneraRosita Angela CondorelliAldo Eugenio CalogeroPublished in: International journal of molecular sciences (2023)
This systematic review and meta-analysis summarize the difference in the methylation of the H19 gene in patients with abnormal versus normal conventional sperm parameters. It also evaluates the effects of age and sperm concentration on H19 methylation in spermatozoa using meta-regression analysis. It was performed according to the MOOSE guidelines for meta-analyses and Systematic Reviews of Observational Studies and the Preferred Reporting Items for Systematic Review and Meta-Analysis Protocols (PRISMA-P). The quality of the evidence reported in the studies included was assessed using the Cambridge Quality Checklists. A total of 11 articles met our inclusion criteria. Quantitative analysis showed that H19 methylation levels were significantly lower in the group of infertile patients than in fertile controls. The reduction in methylation was much more pronounced in patients with oligozoospermia (alone or associated with other sperm parameter abnormalities) and in those with recurrent pregnancy loss. Meta-regression analysis showed the results to be independent of both patient age and sperm concentration. Therefore, the H19 methylation pattern should be evaluated among couples accessing assisted reproductive techniques (ART), in order to gain prognostic information on ART outcome and offspring health.
Keyphrases
- genome wide
- dna methylation
- meta analyses
- systematic review
- end stage renal disease
- healthcare
- type diabetes
- public health
- chronic kidney disease
- ejection fraction
- newly diagnosed
- mental health
- copy number
- gene expression
- preterm birth
- adverse drug
- adipose tissue
- emergency department
- health information
- pregnant women
- risk assessment
- high fat diet
- polycystic ovary syndrome
- tyrosine kinase
- climate change
- case report
- peritoneal dialysis
- antiretroviral therapy
- social media
- skeletal muscle
- transcription factor
- genome wide identification