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New manual qPCR assay validated on tongue swabs collected and processed in Uganda shows sensitivity that rivals sputum-based molecular TB diagnostics.

Amy SteadmanAlfred AndamaAlexey BallJob MukwatamunduKhushboo KhimaniTessa MochizukiLucy AsegeAlice BukirwaJohn Baptist KatoDavid KatumbaEsther KisakyeWilson MangeniSandra MwebeMartha NakayeIrene NassunaJustine NyawereAnnet NakaweesaCatherine CookPatrick Peter John PhillipsTalemwa NalugwaChristine M BachmanFred Collins SemitalaBernhard H WeiglJohn T ConnellyWilliam WorodriaAdithya Cattamanchi
Published in: Clinical infectious diseases : an official publication of the Infectious Diseases Society of America (2024)
Tongue swabs are a promising alternative to sputum for molecular diagnosis of TB, with sensitivity approaching sputum-based molecular tests. Our results provide valuable insights for developing successful tongue swab-based TB diagnostics.
Keyphrases
  • mycobacterium tuberculosis
  • cystic fibrosis
  • pulmonary tuberculosis