Packaging and delivering enzymes by amorphous metal-organic frameworks.
Xiaoling WuHua YueYuanyu ZhangXiaoyong GaoXiaoyang LiLicheng WangYufei CaoMiao HouHaixia AnLin ZhangSai LiJingyuan MaHe LinYanan FuHongkai GuWen-Yong LouWei WeiRichard N ZareJun GePublished in: Nature communications (2019)
Enzymatic catalysis in living cells enables the in-situ detection of cellular metabolites in single cells, which could contribute to early diagnosis of diseases. In this study, enzyme is packaged in amorphous metal-organic frameworks (MOFs) via a one-pot co-precipitation process under ambient conditions, exhibiting 5-20 times higher apparent activity than when the enzyme is encapsulated in corresponding crystalline MOFs. Molecular simulation and cryo-electron tomography (Cryo-ET) combined with other techniques demonstrate that the mesopores generated in this disordered and fuzzy structure endow the packaged enzyme with high enzyme activity. The highly active glucose oxidase delivered by the amorphous MOF nanoparticles allows the noninvasive and facile measurement of glucose in single living cells, which can be used to distinguish between cancerous and normal cells.
Keyphrases
- metal organic framework
- living cells
- fluorescent probe
- induced apoptosis
- single molecule
- room temperature
- cell cycle arrest
- electron microscopy
- high resolution
- air pollution
- oxidative stress
- cell death
- particulate matter
- hydrogen peroxide
- magnetic resonance imaging
- skeletal muscle
- cell proliferation
- solid state
- blood pressure
- neural network
- virtual reality