Activators of SIRT1 in wound repair: an animal model study.

Caloric restriction (CR) and resveratrol activate SIRT1 and induce anti-inflammatory and antioxidant properties. We perform excisional lesion on the dorsum of four groups anesthetized animals: ad libitum-AL diet, AL diet with topical application of 2% resveratrol-Rv, 30% calorie restricted, and finally 30% calorie restricted with 2% resveratrol and we examine CR and Rv effects in wound repair. Restricted animals remained with CR for 31 days. The lesion was performed on day 18 of CR, and resveratrol application was started on day 19. Lesion samples were then collected on days 3 and 10 of treatment for structural, morphometric, and protein analyses. Our results showed that CR and Rv group as well as R group had enhanced numbers of blood vessels, VEGF, fibroblast, birefringent collagen fiber areas in the lesion. We conclude that effects in wound repair suggests that both CR and resveratrol may modulate angiogenesis, fibroplasia, and collagenesis, which could be ascribed to the action of SIRT1.