Recent Advances in Quenchbody, a Fluorescent Immunosensor.
Jinhua DongHiroshi UedaPublished in: Sensors (Basel, Switzerland) (2021)
The detection of viruses, disease biomarkers, physiologically active substances, drugs, and chemicals is of great significance in many areas of our lives. Immunodetection technology is based on the specificity and affinity of antigen-antibody reactions. Compared with other analytical methods such as liquid chromatography coupled with mass spectrometry, which requires a large and expensive instrument, immunodetection has the advantages of simplicity and good selectivity and is thus widely used in disease diagnosis and food/environmental monitoring. Quenchbody (Q-body), a new type of fluorescent immunosensor, is an antibody fragment labeled with fluorescent dyes. When the Q-body binds to its antigen, the fluorescence intensity increases. The detection of antigens by changes in fluorescence intensity is simple, easy to operate, and highly sensitive. This review comprehensively discusses the principle, construction, application, and current progress related to Q-bodies.
Keyphrases
- label free
- liquid chromatography
- mass spectrometry
- living cells
- high resolution mass spectrometry
- tandem mass spectrometry
- quantum dots
- single molecule
- high intensity
- capillary electrophoresis
- simultaneous determination
- energy transfer
- high performance liquid chromatography
- dendritic cells
- high resolution
- gas chromatography
- fluorescent probe
- solid phase extraction
- computed tomography
- risk assessment
- patient reported outcomes
- ms ms