The first report of animal genotypes of Cryptosporidium parvum in immunosuppressed and immunocompetent humans in Slovakia.
Elena HatalováAlexandra ValenčákováLenka LuptákováMichaela ŠpalkováJana KalinováMonika HalánováVeronika BednárováJuliana GabzdilováKinga DedinskáFrantišek OndriskaVojtech BoldišPublished in: Transboundary and emerging diseases (2018)
The aim of our study was to determine species and genotypes of Cryptosporidium in patients suffering from immunosuppressive illnesses, but also in immunocompetent patients suffering from diarrhoea. A total of 80 samples of faeces were collected from both immunosuppressed and immunocompetent patients. The immunosuppressed patients (65 samples) - 35 adult patients (group A) and 30 children (group B) were hospitalized at the Clinic of Oncohemathology. Samples from immunocompetent humans (15 samples, group C) were taken from patients with clinical signs of acute diarrhoea. With the use of molecular methods targeting the 60 kDa glycoprotein (GP60) gene region, we have identified multiple genotypes of Cryptosporidium. parvum and Cryptosporidium. hominis in immunocompromised, but also in immunocompetent individuals (C. hominis IbA10G2, IeA12G3T3; C. parvum IIaA10G1R1, IIaA11G2R1, IIaA12G2R1, IIaA13G1R1, IIaA14G1R1, IIaA14G2R1, IIaA17G1R1 and IIaA18G1R1). This is the first report of the occurrence of genotypes IIaA10G1R1, IIa12G2R1 and IIaA18G1R1 in human hosts.
Keyphrases
- end stage renal disease
- newly diagnosed
- chronic kidney disease
- peritoneal dialysis
- risk assessment
- primary care
- endothelial cells
- gene expression
- young adults
- intensive care unit
- drug delivery
- transcription factor
- hepatitis b virus
- acute respiratory distress syndrome
- dna methylation
- liver failure
- patient reported
- genome wide
- single molecule