The growth factors amphiregulin and epiregulin are novel stimulators of feline ovarian granulosa cell functions.
Zuzana FabováBarbora LoncováAbdel Halim HarrathAlexander V SirotkinPublished in: Reproduction in domestic animals = Zuchthygiene (2024)
This study aimed to investigate the impact of the epidermal growth factor receptor ligands amphiregulin (AREG) and epiregulin (EREG) on the fundamental functions of feline ovarian granulosa cells. Granulosa cells isolated from feline ovaries were incubated with AREG and EREG (0, 0.1, 1 or 10 ng/mL). The effects of these growth factors on cell viability, proliferation (assessed through BrdU incorporation), nuclear apoptosis (evaluated through nuclear DNA fragmentation) and the release of progesterone and estradiol were determined using Cell Counting Kit-8 assays, BrdU analysis, TUNEL assays and ELISAs, respectively. Both AREG and EREG increased cell viability, proliferation and steroid hormone release and reduced apoptosis. The present findings suggest that these epidermal growth factor receptor ligands may serve as physiological stimulators of feline ovarian cell functions.
Keyphrases
- epidermal growth factor receptor
- cell cycle arrest
- induced apoptosis
- tyrosine kinase
- advanced non small cell lung cancer
- single cell
- cell death
- endoplasmic reticulum stress
- cell therapy
- oxidative stress
- signaling pathway
- type diabetes
- adipose tissue
- metabolic syndrome
- single molecule
- circulating tumor
- mesenchymal stem cells
- cell proliferation
- insulin resistance