Determination of cytogenetic markers for biological monitoring in coypu (Myocastor coypu).
Marta Kuchta-GładyszEwa WójcikDorota SłoninaAnna GrzesiakowskaAgnieszka Otwinowska-MindurOlga SzeleszczukPiotr NiedbałaPublished in: Animal science journal = Nihon chikusan Gakkaiho (2020)
Cytogenetic tests are used to assess the influence of physical and chemical factors with potential mutagenic and genotoxic properties on the animal organism. The test results make it possible to eliminate mutagens, as well as helping predict possible genetic consequences in animal cells and assess animal resistance. The aim of this study was to examine, using cytogenetic tests, the spontaneous chromosome and DNA damage in coypu lymphocytes. Four tests: fragile site (FS), bleomycin (BLM), micronucleus, (MN) and comet were used for the first time in coypu cells. The averages with standard deviations obtained in the research were as follows: 3.30 ± 0.80 fragile sites/cell; 0.63 ± 0.80 BLM damage/cell; 6.10 ± 0.53% binucleated cells with MN; and 3.24 ± 0.63% DNA in tail. The present analysis showed high interindividual variation in spontaneous chromosomal and DNA damage levels. In the case of micronucleus, fragile sites, and comet assays, the differences between animals were statistically significant. The data suggest that these assays are sensitive enough to detect some effects on an individual animal and can be proposed as tools for coypu biomonitoring.
Keyphrases
- dna damage
- induced apoptosis
- oxidative stress
- cell cycle arrest
- endoplasmic reticulum stress
- single cell
- copy number
- physical activity
- cell therapy
- signaling pathway
- stem cells
- cell death
- high throughput
- electronic health record
- dna methylation
- mass spectrometry
- machine learning
- deep learning
- circulating tumor
- pi k akt
- bone marrow
- ionic liquid
- high resolution