DNA photo-cross-linking using a pyranocarbazole-modified oligodeoxynucleotide with a d-threoninol linker.

An alternative photo-cross-linker having a d-threoninol skeleton instead of the 2'-deoxyribose backbone in 3-cyanovinylcarbazole ( CNV K) was investigated to improve the photoreactivity of photo-cross-linkers; the photo-cross-linking rate of 3-cyanovinylcarbazole with d-threoninol ( CNV D) was found to be greater than that of CNV K. Therefore, in this study, a novel photo-cross-linker having pyranocarbazole ( PC X) and d-threoninol instead of the 2'-deoxyribose backbone in PC X ( PCX D) was developed. The PCX D in double-stranded DNA photo-cross-linked to a pyrimidine base at the -1 position of a complementary strand similar to PC X. Furthermore, the photoreactivity of PCX D was significantly higher than that of PC X. The introduction of d-threoninol improved the reactivity of pyranocarbazole to cytosine, the use of PCX D may extend the applicability of the photo-cross-linking reaction for DNA manipulation. In particular, this novel photo-cross-linker can contribute to the photochemical regulation of gene expression or biological events in a living cell.