Ultrasensitive pathogen detection with a rolling circle amplification-empowered multiplex electrochemical DNA sensor.
Cheryl S Y YeapThanyarat ChaibunSu Yin LeeBin ZhaoYuan JanChan La-O-VorakiatWerasak SurareungchaiShiping SongBenchaporn LertanantawongPublished in: Chemical communications (Cambridge, England) (2021)
We report a highly sensitive and selective multiplex assay by empowering an electrochemical DNA sensor with isothermal rolling circle amplification. The assay could simultaneously detect and discriminate three common entero-pathogens in a single reaction, with femtomolar sensitivity. It is useful for field- or resource-limited settings.
Keyphrases
- label free
- high throughput
- nucleic acid
- circulating tumor
- molecularly imprinted
- real time pcr
- gold nanoparticles
- cell free
- single molecule
- single cell
- candida albicans
- antimicrobial resistance
- gram negative
- solid phase extraction
- ionic liquid
- mass spectrometry
- electron transfer
- loop mediated isothermal amplification
- sensitive detection