Detection of invasive Bartonella infections with next-generation sequencing of microbial cell-free DNA.

Fernando H Centeno Ahmed M HamdiTodd M LascoMayar Al Mohajer

Published in: Antimicrobial stewardship & healthcare epidemiology : ASHE (2024)

We report 9 patients with invasive Bartonella infections, including 5 with endocarditis, who were diagnosed with microbial cell-free DNA next-generation sequencing and Bartonella serology studies. Diagnosis with plasma mcfDNA NGS enabled a faster clinical and laboratory diagnosis in 8 patients. Prompt diagnosis impacted antibiotic management in all 9 patients.

Keyphrases

end stage renal disease
ejection fraction
newly diagnosed
peritoneal dialysis
prognostic factors
microbial community
copy number
dna methylation
loop mediated isothermal amplification