Specific N-terminal attachment of TMTHSI linkers to native peptides and proteins for strain-promoted azide alkyne cycloaddition.
Matt TimmersWouter PeetersNiels J HauwertCristianne J F RijckenTina VermondenIngrid DijkgraafRob M J LiskampPublished in: Chemical communications (Cambridge, England) (2023)
The site specific attachment of the reactive TMTHSI-click handle to the N-terminus of peptides and proteins is described. The resulting molecular constructs can be used in strain-promoted azide alkyne cycloaddition (SPAAC) for reaction with azide containing proteins e.g. , antibodies, peptides, nanoparticles, fluorescent dyes, chelators for radioactive isotopes and SPR-chips etc .
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