Co-Administration of Nanowired Monoclonal Antibodies to Inducible Nitric Oxide Synthase and Tumor Necrosis Factor Alpha Together with Antioxidant H-290/51 Reduces SiO 2 Nanoparticles-Induced Exacerbation of Pathophysiology of Spinal Cord Trauma.
Aruna SharmaDafin F MuresanuZ Ryan TianAla NozariJosé Vicente LafuenteAnca D BuzoianuPer-Ove SjöquistLianyuan FengLars WiklundHari Shanker SharmaPublished in: Advances in neurobiology (2023)
Military personnel are often exposed to silica dust during combat operations across the globe. Exposure to silica dust in US military or service personnel could cause Desert Strom Pneumonitis also referred to as Al Eskan disease causing several organs damage and precipitate autoimmune dysfunction. However, the effects of microfine particles of sand inhalation-induced brain damage on the pathophysiology of traumatic brain or spinal cord injury are not explored. Previously intoxication of silica nanoparticles (50-60 nm size) is shown to exacerbates spinal cord injury induces blood-spinal cord barrier breakdown, edema formation and cellular changes. However, the mechanism of silica nanoparticles-induced cord pathology is still not well known. Spinal cord injury is well known to alter serotonin (5-hydroxytryptamine) metabolism and induce oxidative stress including upregulation of nitric oxide synthase and tumor necrosis factor alpha. This suggests that these agents are involved in the pathophysiology of spinal cord injury. In this review, we examined the effects of combined nanowired delivery of monoclonal antibodies to neuronal nitric oxide synthase (nNOS) together with tumor necrosis factor alpha (TNF-α) antibodies and a potent antioxidant H-290/51 to induce neuroprotection in spinal cord injury associated with silica nanoparticles intoxication. Our results for the first time show that co-administration of nanowired delivery of antibodies to nNOS and TNF-α with H-290/51 significantly attenuated silica nanoparticles-induced exacerbation of spinal cord pathology, not reported earlier.
Keyphrases
- spinal cord injury
- nitric oxide synthase
- spinal cord
- oxidative stress
- diabetic rats
- nitric oxide
- neuropathic pain
- rheumatoid arthritis
- high glucose
- chronic obstructive pulmonary disease
- health risk
- cell proliferation
- dna damage
- white matter
- multiple sclerosis
- anti inflammatory
- walled carbon nanotubes
- induced apoptosis
- systemic sclerosis
- interstitial lung disease
- signaling pathway
- ischemia reperfusion injury
- posttraumatic stress disorder
- blood brain barrier
- long non coding rna