Differences in PPD- and mitogen-induced T-cell activation marker expression characterize immunopathology in acute tuberculosis patients.
Isaac AcheampongDifery MinadziEdwin F LaingMichael FrimpongMonika M VivekanandanAugustine YeboahErnest AdankwahWilfred AniagyeiJoseph F ArthurMillicent LampteyMohammed K AbassFrancis KumbelFrancis Osei-YeboahAmidu GawusuLinda Batsa DebrahDorcas O OwusuAlexander DebrahErtan MayatepekJulia SeyfarthRichard O PhillipsMarc JacobsenPublished in: European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology (2024)
Impaired T-cell responses to mitogens and high T-cell activation marker (TAM) expression on Mycobacterium tuberculosis-specific T-cells characterize immunopathology in patients with tuberculosis (TB). In a study of patients with TB (n = 60) and asymptomatic contacts (controls, n = 37), we found that TB patients had higher CD38 + T-cell proportions specific for M. tuberculosis protein (PPD Mtb ), yet total proportions of PPD Mtb -specific T-cells were comparable. Notably, both activated (CD38 + ) and total IFN-γ + T-cells from TB patients had lower mitogen (phytohemagglutinin, PHA)-induced responses. This impaired mitogen response improved the classification efficacy of the TAM-TB assay, especially employing the PPD/PHA-induced T-cell ratio.
Keyphrases
- mycobacterium tuberculosis
- end stage renal disease
- pulmonary tuberculosis
- newly diagnosed
- ejection fraction
- chronic kidney disease
- machine learning
- peritoneal dialysis
- prognostic factors
- emergency department
- high glucose
- high throughput
- protein kinase
- intensive care unit
- drug induced
- small molecule
- immune response
- inflammatory response
- hepatitis c virus
- binding protein
- toll like receptor
- endothelial cells
- respiratory failure