Neuropsychiatric Behavioral Assessments in Mice After Acute and Long-Term Treatments of Low-Intensity Pulsed Ultrasound.
Ye LiYiqing WuQi LuoXuanjie YeJie ChenYuanlin SuKe ZhaoXinmin LiJing LinZhiqian TongQi WangDongwu XuPublished in: American journal of Alzheimer's disease and other dementias (2024)
Introduction: To evaluate whether both acute and chronic low-intensity pulsed ultrasound (LIPUS) affect brain functions of healthy male and female mice. Methods: Ultrasound (frequency: 1.5 MHz; pulse: 1.0 kHz; spatial average temporal average (SATA) intensity: 25 mW/cm 2 ; and pulse duty cycle: 20%) was applied at mouse head in acute test for 20 minutes, and in chronic experiment for consecutive 10 days, respectively. Behaviors were then evaluated. Results: Both acute and chronic LIPUS at 25 mW/cm 2 exposure did not affect the abilities of movements, mating, social interaction, and anxiety-like behaviors in the male and female mice. However, physical restraint caused struggle-like behaviors and short-time memory deficits in chronic LIPUS groups in the male mice. Conclusion: LIPUS at 25 mW/cm 2 itself does not affect brain functions, while physical restraint for LIPUS therapy elicits struggle-like behaviors in the male mice. An unbound helmet targeted with ultrasound intensity at 25-50 mW/cm 2 is proposed for clinical brain disease therapy.
Keyphrases
- liver failure
- magnetic resonance imaging
- drug induced
- respiratory failure
- blood pressure
- resting state
- high fat diet induced
- white matter
- physical activity
- mental health
- healthcare
- traumatic brain injury
- high intensity
- ultrasound guided
- cerebral ischemia
- computed tomography
- contrast enhanced ultrasound
- high frequency
- hepatitis b virus
- functional connectivity
- stem cells
- extracorporeal membrane oxygenation
- multiple sclerosis
- drug delivery
- intensive care unit
- metabolic syndrome
- working memory
- skeletal muscle
- depressive symptoms
- mechanical ventilation
- obstructive sleep apnea
- subarachnoid hemorrhage
- wild type
- smoking cessation
- sleep apnea