Multiplexed Biosensing of Proteins and Virions with Disposable Plasmonic Assays.
Stephanie WallaceMartin KartauTarun KakkarChris DavisAgnieszka SzemielIliyana SamardzhievaSwetha VijayakrishnanSarah ColeGiuditta De LorenzoEmmanuel MaillartKevin GautierAdrian J LapthornArvind H PatelNikolaj GadegaardMalcolm KadodwalaEdward HutchinsonAffar S KarimullahPublished in: ACS sensors (2023)
Our growing ability to tailor healthcare to the needs of individuals has the potential to transform clinical treatment. However, the measurement of multiple biomarkers to inform clinical decisions requires rapid, effective, and affordable diagnostics. Chronic diseases and rapidly evolving pathogens in a larger population have also escalated the need for improved diagnostic capabilities. Current chemical diagnostics are often performed in centralized facilities and are still dependent on multiple steps, molecular labeling, and detailed analysis, causing the result turnaround time to be over hours and days. Rapid diagnostic kits based on lateral flow devices can return results quickly but are only capable of detecting a handful of pathogens or markers. Herein, we present the use of disposable plasmonics with chiroptical nanostructures as a platform for low-cost, label-free optical biosensing with multiplexing and without the need for flow systems often required in current optical biosensors. We showcase the detection of SARS-CoV-2 in complex media as well as an assay for the Norovirus and Zika virus as an early developmental milestone toward high-throughput, single-step diagnostic kits for differential diagnosis of multiple respiratory viruses and any other emerging diagnostic needs. Diagnostics based on this platform, which we term "disposable plasmonics assays," would be suitable for low-cost screening of multiple pathogens or biomarkers in a near-point-of-care setting.
Keyphrases
- label free
- high throughput
- low cost
- zika virus
- healthcare
- sars cov
- single cell
- gram negative
- loop mediated isothermal amplification
- dengue virus
- high resolution
- high speed
- risk assessment
- preterm infants
- multidrug resistant
- aedes aegypti
- climate change
- human health
- sensitive detection
- energy transfer
- real time pcr
- replacement therapy
- smoking cessation