Templated Self-Assembly of a Covalent Polymer Network for Intracellular Protein Delivery and Traceless Release.
Kingshuk DuttaDing HuBo ZhaoAlexander E RibbeJiaming ZhuangSankaran ThayumanavanPublished in: Journal of the American Chemical Society (2017)
Trafficking proteins inside cells is an emerging field with potential utility in basic cell biology and biological therapeutics. A robust and sustainable delivery strategy demands not only good protection of the cargo but also reversibility in conjugation and activity. We report a protein-templated polymer self-assembly strategy for forming a sheath around the proteins and then tracelessly releasing them in the cytosol. The versatility of the approach, demonstrated here, suggests that the strategy is compatible with a wide array of biologics.